當前位置:廣州健侖生物科技有限公司>>生物試劑>>檢測試劑>> 登革熱IgG抗體檢測登革熱病毒IgG抗體檢測試劑盒
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登革熱IgG抗體檢測登革熱病毒IgG抗體檢測試劑盒
廣州健侖生物科技有限公司
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登革熱IgM抗體、登革熱IgG抗體、登革熱NS1抗原、登革熱早期檢測試劑盒
進口登革熱檢測卡 登革熱病毒檢測試劑盒
美國NovaBios登革熱IgG抗體檢測登革熱病毒IgG抗體檢測試劑盒
在發病的早期(發病5天內)一般采用病毒分離、核酸檢測或抗原檢測等方法進行診斷,當病程進入恢復期后(發病5天以后),一般采用血清學檢測病毒特異性抗體的方法進行診斷。
1. 病毒分離。經細胞培養分離病毒,需要生物安全二級(BSL-2)實驗室及必要的儀器設備,在標本運輸過程中保持低溫(冷凍或冷藏)對于病毒分離非常重要。將標本接種于蚊源細胞(C6/36)或哺乳動物細胞(BHK21、Vero)進行分離培養,出現病變以后,用檢測抗原或核酸的方法鑒定病毒。分離到登革病毒可以確診,但其耗時長,需要數天,不適于快速診斷。
2. 核酸檢測。多種逆轉錄聚合酶鏈反應(RT-PCR)方法可用于登革病毒核酸檢測,包括一步法RT-PCR、實時熒光RT-PCR或套式RT-PCR等方法等。核酸檢測可在1~2天內鑒別病毒RNA。在病人標本中檢出病毒核酸,可確診且能分型,可用于早期診斷,但核酸檢測容易因污染而產生假陽性,因此要求嚴格分區操作。陰性結果不能排除登革熱診斷,需采集第二份標本(發病5天后)開展血清學檢測,進行確診。
美國NovaBios登革熱IgG抗體檢測登革熱病毒IgG抗體檢測試劑盒
Detection method selection
In the early onset (within 5 days of onset) generally used virus isolation, nucleic acid detection or antigen detection and other methods for diagnosis, when the disease into the recovery period (5 days after the onset), the general use of serological detection of virus-specific antibody method for diagnosis The
1. virus separation. It is important to isolate the virus by cell culture, require a biosafety two (BSL-2) laboratory and the necessary equipment to keep the cryogenic (frozen or frozen) during the transport of the specimen. The specimens were inoculated in mosquito-borne cells (C6 / 36) or mammalian cells (BHK21, Vero) for isolation and culture. After the lesion, the virus was identified by detecting antigen or nucleic acid. Separation of dengue virus can be diagnosed, but it takes a long time, takes several days, is not suitable for rapid diagnosis.
2. nucleic acid detection. A variety of reverse transcription polymerase chain reaction (RT-PCR) methods can be used for dengue virus nucleic acid detection, including one-step RT-PCR, real-time fluorescence RT-PCR or nested RT-PCR and other methods. Nucleic acid detection can identify viral RNA within 1 to 2 days. Virus nucleic acid is detected in the patient's specimen. It can be diagnosed and can be classified and can be used for early diagnosis. However, nucleic acid detection is prone to false positives due to pollution. Therefore, strict zoning is required. Negative results can not rule out dengue diagnosis, need to collect the second specimen (5 days after the onset) to carry out serological tests, for diagnosis.
3. Antigen detection. NS1 antigen detection can be used ELISA method or rapid detection reagent, can be completed in the tens of minutes to several hours of detection, suitable for on-site use, dengue fever is an important means of acute diagnosis, can be detected 1 day after the onset, there are reports in 18 days after onset can still be detected in blood samples. At present the method can not be classified. Due to the specificity of the NS1 antigen detection method, it can also be used for the differential diagnosis of flavivirus infection. Based on the characteristics of China's dengue epidemic, the National Health Commission, "dengue fever diagnosis and treatment guidelines (the second edition of 2014)" specimens detected NS1 antigen as a basis for the diagnosis of viral infection can be used for early diagnosis. Negative results can not rule out dengue diagnosis, need to collect the second specimen (5 days after the onset) to carry out serological tests, for diagnosis.
美國NovaBios登革熱IgG抗體檢測登革熱病毒IgG抗體檢測試劑盒
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【公司名稱】 廣州健侖生物科技有限公司
【市場部】 楊永漢
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【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室
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