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登革病毒IgG抗體登革病毒IgG抗體診斷試劑盒
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美國NovaBios登革病毒IgG抗體登革病毒IgG抗體診斷試劑盒
乳鼠分離登革熱病毒
標本:
病毒分離:
(1)每一檢材接種一窩1~2日齡乳鼠,腦內接種0.01ml/只。接種后48小時內死亡者按非特異性死亡處理,棄去。
(2)存活者觀察10天,若仍未發病,則解剖其中2只,取腦,用pH8.0肉湯制成10%懸液,盲傳一窩1~2日齡乳鼠,存活鼠和盲傳鼠均觀察到第4周,未發病為陰性結果;
(3)在觀察期內發病(活動力降低、站立不穩、肢體抽搐、不全麻痹等癥狀)者,解剖,取半邊腦,按盲傳法制成10%懸液,接種一窩1~2日齡乳鼠,并作無菌試驗;另半邊腦置5.43mol/L(50%)甘油緩沖液中低溫保存。無菌試驗陰性而重復以上癥狀者作為可以毒種傳代、保存,并鑒定。
結果判斷:
細胞內病毒特異性熒光為黃綠色顆粒,分布在感染細胞的胞漿內。根據特異性熒光顆粒多少、熒光亮度、陽性細胞在細胞總數中所占比例,可將免疫熒光反應大致區分為1~4個“+”。
檢測抗體滴度時,以能觀察到明顯特異性熒光反應(>“+”)zui高血清稀釋度的倒數表示。
意義:
陽性結果,表明曾受到DV感染,>1:80有診斷參考意義;
恢復期血清抗體滴度比急性期抗體滴度有4倍或4倍以上升高則可確診。
美國NovaBios登革病毒IgG抗體登革病毒IgG抗體診斷試劑盒
Separation of dengue virus in neonatal rats
Specimen:
Virus isolation:
In the case of
(1) each sample inoculated with a nest 1 to 2 days old suckling, brain inoculation 0.01ml / only. Within 48 hours after inoculation, the deathees were treated with non-specific death and discarded. In the case of
In the case of
(2) survivors observed 10 days, if not yet disease, then anatomy of which 2, take the brain, with pH8 broth made 10% suspension, blind pass 1 to 2 days old piglets, And the blinds were observed in the first 4 weeks, no incidence of negative results;
(3) in the observation period of the disease (decreased mobility, standing instability, limb convulsions, incomplete paralysis and other symptoms), anatomy, take half of the brain, according to blind pass made 10% suspension, inoculation of a nest 1 to 2 days And 504% glycerol buffer in low temperature preservation. The aseptic test was negative and repeated the above symptoms as the virus can be passaged, preserved, and identified.
Judgment:
Intracellular virus-specific fluorescence is yellow-green particles, distributed in the cytoplasm of infected cells. According to the number of specific fluorescent particles, fluorescence brightness, the proportion of positive cells in the total number of cells, immunofluorescence reaction can be roughly divided into 1 to 4 "+".
When the antibody titer was detected, the reciprocal of the highest serum dilution was observed with a significant specific fluorescence response (& gt; + & gt;).
Meaning:
Positive results, indicating that had been infected with DV,> 1:80 has a diagnostic reference;
Recovery serum antibody titer than the acute phase antibody titer 4 times or more than 4 times higher can be diagnosed.
美國NovaBios登革病毒IgG抗體
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