RmesFect™ Stem Transfection Reagent is based on the TEE-technology specifically designed for mRNA transfection in Stem Cells with high efficiency and low toxicity. mRNA transfection provides several advantages over plasmid DNA delivery :

No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm

Faster protein expression than DNA transfection

No genomic integration

Protein expression in a total promoter independent manner

RmesFect™Stem is specifically designed for in vitro mRNA (dsRNA) transfection in a large variety stem cells and primary cells.

RmesFect™Stem transfection reagent principal advantages:

Highly efficient : achieve mRNA delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects mRNA against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

Sizes:

500µL: 125-250 transfections with 1 µg of mRNA

1 mL: 250-500 transfections with 1 µg of mRNA

5 mL: 1250-2500 transfections with 1 µg of mRNA

艾美捷OZ Biosciences RmesFect莖轉(zhuǎn)染試劑基于三通技術(shù) 專為干細(xì)胞中的mRNA轉(zhuǎn)染而設(shè)計(jì)，具有高效率和低毒性。與質(zhì)粒DNA遞送相比，mRNA轉(zhuǎn)染具有以下優(yōu)勢(shì)：

1、無需核吸收。mRNA翻譯成蛋白質(zhì)發(fā)生在細(xì)胞質(zhì)中

2、蛋白表達(dá)速度快于DNA轉(zhuǎn)染

3、無基因組整合

4、以完-全啟動(dòng)子獨(dú)立的方式表達(dá)蛋白質(zhì)

OZ Biosciences RmesFect莖轉(zhuǎn)染試劑專為多種干細(xì)胞和原代細(xì)胞中的體外mRNA （dsRNA）轉(zhuǎn)染而設(shè)計(jì)。

規(guī)格：

500μL：用 125 μg mRNA 轉(zhuǎn)染 250-1 次

1 mL：用 250 μg mRNA 轉(zhuǎn)染 500-1 次

5 mL：用 1250 μg mRNA 轉(zhuǎn)染 2500-1 次

OZ Biosciences RmesFect莖轉(zhuǎn)染試劑主要優(yōu)點(diǎn)：

1、高效：在所有細(xì)胞中實(shí)現(xiàn)mRNA遞送

2、即用型：無需額外的緩沖液

3、低核酸量：毒性最小化

4、保護(hù) mRNA 免受降解

5、與任何培養(yǎng)基兼容

6、血清兼容：無需更換培養(yǎng)基

OZ Biosciences RmesFect莖轉(zhuǎn)染試劑結(jié)果：

圖1：使用RmesFect Stem轉(zhuǎn)染了幾個(gè)干細(xì)胞。復(fù)合物的制備方法如下：將編碼GFP蛋白的mRNA與RmesFect Stem轉(zhuǎn)染試劑（hMSC為0：25，MEF和hAdSC為0：5）混合編碼GFP蛋白（人hMSC為2.1 μg，MEF和hAdSC為4.1 μg）。在室溫下孵育20分鐘后，將復(fù)合物以滴加方式添加到細(xì)胞中。24 h后，通過FACS分析測(cè)量轉(zhuǎn)染效率。

圖2：使用RmesFect干細(xì)胞轉(zhuǎn)染了幾個(gè)干細(xì)胞。24 h后，通過熒光顯微鏡測(cè)量轉(zhuǎn)染效率。