RmesFect™ CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency and low toxicity. RmesFect™ is efficient in a large variety of cells.

Benefits:

Highly efficient: achieve RNAs delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects RNAs against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

mRNA transfection provides several advantages over plasmid DNA delivery :

No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm.

Faster protein expression than DNA transfection

No genomic integration

Protein expression in a total promoter-independent manner

Additional products for CRISPR Cas9 experiments:

PolyMag CRISPR for Genome editing using expression plasmids

ProDeliverIN CRISPR for Cas9 protein delivery

ViroMag CRISPR to enhance transduction efficiency of CRISPR/Cas9 viruses

基于TEE技術的艾美捷 RmesFect™ CRISPR轉染試劑專為mRNA/gRNA轉染而設計，具有高效率和低毒性的特點。RmesFect™在多種細胞中都是有效的。

OZ Biosciences RmesFect CRISPR轉染試劑特點：

高效：在所有細胞中實現(xiàn)RNA遞送

即用型：無需額外的緩沖液

低核酸量：毒性最小化

保護 RNA 免受降解

與任何培養(yǎng)基兼容

血清兼容：無需更換培養(yǎng)基

圖 1：根據(jù)標準方案，使用 RmesFect CRISPR 轉染編碼 GFP 和 RFP（各 24.0μg）的 mRNA 轉染 DC25 細胞。轉染后24h在顯微鏡熒光下分析圖像。

與質(zhì)粒DNA遞送相比，mRNA轉染具有以下優(yōu)勢：

無需核吸收。mRNA翻譯成蛋白質(zhì)發(fā)生在細胞質(zhì)中。

蛋白表達速度快于DNA轉染

無基因組整合

以完-全啟動子非依賴的方式表達蛋白質(zhì)

用于CRISPR Cas9實驗的其他產(chǎn)品：

PolyMag CRISPR用于使用表達質(zhì)粒進行基因組編輯

ProDeliverIN CRISPR用于 Cas9 蛋白遞送

ViroMag CRISPR提高CRISPR/Cas9病毒的轉導效率