Calcofluor white dye is a fluorescent blue dye that can bind to cellulose and chitin, which are present in the cell walls of fungi, algae, and plants. In budding yeast, the Calcofluor white stain stains the bud scars more strongly because they contain higher concentrations of chitin. Calcofluor white staining is sometimes used to identify fungal infections in medical samples.

Calcofluor白色染色劑是一種熒光藍(lán)色染料，可與纖維素和幾丁質(zhì)結(jié)合，后者存在于真菌、藻類和植物的細(xì)胞壁中。在出芽酵母中，Calcofluor 白色染色劑對芽痕的染色更強(qiáng)烈，因?yàn)樗鼈兒懈邼舛鹊膸锥≠|(zhì)。Calcofluor 白色染色劑 有時(shí)用于識別醫(yī)學(xué)樣本中的真菌感染。

液體培養(yǎng)細(xì)胞染色方案：

該染色方案使用釀酒酵母進(jìn)行了優(yōu)化在純粹的文化中。可能需要確定合適染料濃度對其他生物體進(jìn)行實(shí)驗(yàn)。

1.在適當(dāng)?shù)纳L培養(yǎng)基中培養(yǎng)細(xì)胞。可選：降速和再懸浮在適合成像的透明緩沖液中，例如PBS或HBSS。

2.將染料以最終濃度直接添加到生長培養(yǎng)基或緩沖液中的細(xì)胞中為5-25μM。

3.在室溫或30°C下培養(yǎng)20分鐘，搖動，防止光可選：成像前清洗一次。

4. 使用DAPI發(fā)射濾光片在熒光顯微鏡上成像。

艾美捷Calcofluor白色染色劑#29067屬性：

激發(fā)/發(fā)射：360/430 納米

分子量：916.98

儲存條件：儲存在-10至-35°C，避光

A） 用25μM Calcofluor White染色的釀酒酵母20分鐘，然后使用蔡司LSM700共焦顯微鏡成像DAPI成像設(shè)置。B） 釀酒酵母順序染色用1μM ViaVac紅/綠和25μM Calcofluor白在PBS中20分鐘。使用DAPI和Texas Red®在蔡司LSM700共焦顯微鏡上成像設(shè)置。

Calcofluor白色染色劑相關(guān)研究：

Catalog number      Product   Function

29068      ViaVac  Red/Green,  10  mM  in  DMSO   Yeast vital dye

40077      Thiazole  Orange,  10 mM in  DMSO   Live cell fixable cytoplasmic stain

31062      Yeast Vitality Staining Kit   Calcofluor White to stain the cell wall, ViaVac Red/Green as a vital dye

31063      Yeast Viability Staining Kit   ConA to stain cell walls, Live-or-Dye to stain dead cells

31064      Yeast Fixable Live/Dead Staining Kit   Thiazole Orange to stain all cells, Live-or-Dye to stain dead cells

29015-29020;29058      CFTM   Dye   ConcanavalinA(Con   A)   Cell wall stain in a variety of dye conjugates

29021-29029; 29059;29064      CFM  Dye  Wheat  Germ  Agglutinin  (WGA)   Cell wall bud scar stain in a variety of dye conjugates

32002-32009      Live-or-Dye Fixable Viability Staining Kits   Dead-cell-specific viability stains in a variety of dye colors

23001      EverBrite  TM Mounting  Medium   Wet  set mounting medium for microscopy

23002      EverBrite M Mounting Medium with DAPI   Wet set mounting medium for microscopy containing DAPI