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技術文獻:蚯蚓8-羥基脫氧鳥苷（8-OHdG）ELISA試劑盒引用文獻
閱讀：505 發布時間：2022-7-27| 提 供 商 | 上海恒遠生物科技有限公司 | 資料大小 | 3.6MB |
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【文獻標題】Ecotoxicity evaluation of azoxystrobin on Eisenia fetida in different soils
【作者】Yaqi Xu,Bing Li, Kaixuan Hou,et.al
【作者單位】山東大學（ Universities of Shandon）
【文獻中引用產品】
蚯蚓8-羥基脫氧鳥苷（8-OHdG）ELISA試劑盒
【關鍵詞】Strobilurin fungicide ,Earthworms ,Toxicity evaluation ,Oxidative damage ,Natural soil
【DOI】doi.org/10.1016/j.envres.2020.110705
【影響因子(IF)】8.4
【出版期刊】《Environmental Research》
【產品原文引用】
Reactive oxygen species (ROS) content was determined using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) method (Guo et al., 2015). 2′,7′-dichlorofluorescein is the product of reaction of ROS and DCFH-DA, which can be detected by a fluorescence spectrophotometer (RF-5301PC, Shimadzu, Japan). MDA content was determined according to Han et al. (2014) by evaluating thiobarbituric acid chromogenic reaction. The UV absorbance were read at 532 nm. DNA oxidative damage indicator (8-OHdG) was determined using enzyme linked immunosorbent assay kit (Shanghai Hengyuan Biological Technology Co., Ltd, China). The samples were read at 450 nm by microplate reader (MK3, Thermo Fisher, USA).
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