PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

Benefits:

•High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

•Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

•Optimized for large plasmid DNA containing Cas9, and guide RNA

•Low cell toxicity - enable to start your experiment with less cell

艾美捷POLYMAG CRISPR 200 轉(zhuǎn)染試劑#PNC40200是經(jīng)過(guò)優(yōu)化的磁鐵輔助轉(zhuǎn)染試劑，可提供表達(dá) Cas9 和向?qū)?RNA （gRNA） 的高水平質(zhì)粒 DNA 和/或 mRNA。該試劑基于我們專(zhuān)有的磁感應(yīng)™技術(shù)。納米顆粒由涂有陽(yáng)離子分子的氧化鐵磁芯組成。對(duì)于您的基因編輯實(shí)驗(yàn)，該試劑具有高轉(zhuǎn)染效率，無(wú)毒性。

POLYMAG CRISPR 200 轉(zhuǎn)染試劑特點(diǎn)：

• 即使在原代細(xì)胞和難以轉(zhuǎn)染的細(xì)胞上也能高效遞送 CRISPR/Cas9 系統(tǒng)• 非常適合共轉(zhuǎn)染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

• 針對(duì)含有 Cas9 的大質(zhì)粒 DNA 進(jìn)行了優(yōu)化，并引導(dǎo) RNA

• 低細(xì)胞毒性 - 能夠以更少的細(xì)胞開(kāi)始實(shí)驗(yàn)

POLYMAG CRISPR 200 轉(zhuǎn)染試劑結(jié)果示例：

圖：PolyMag CRISPR共轉(zhuǎn)染效率用于CRISPR-Cas9實(shí)驗(yàn)。將NIH-3t3、HeLa和MDCK細(xì)胞接種在完-全培養(yǎng)基中的24孔板中，轉(zhuǎn)染時(shí)最佳密度為50-70%匯合。用GFP-Cas9質(zhì)粒和RFP pDNA轉(zhuǎn)染細(xì)胞，然后在37oC下孵育過(guò)夜。轉(zhuǎn)染后24h熒光顯微鏡監(jiān)測(cè)GFP和RFP表達(dá)。