In vivo PolyMag™ Transfection Reagent has been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA, oligonucleotides using magnetic nanoparticles coated with specific polymers.  

DNA complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

In vivo Magnetofection™ main advantages in comparison to other procedures:

Increased transfection efficiency

Targeted process (magnetically-driven)

Reduction of the systemic dissemination of vectors during injection

Reduction of the vector doses

Work under non permissive conditions (hypothermia, physiological flow conditions)

Penetration of the vector into tissues

Minimized toxicity

Universal – suitable for all nucleic acids

艾美捷IN VIVO POLYMAG 500 轉(zhuǎn)染試劑#IV-PN30500設計用于使用涂有特定聚合物的磁性納米顆粒對各種類型的核酸（如DNA、RNA、寡核苷酸）進行體內(nèi)靶向轉(zhuǎn)染。

DNA復合物可以通過各種注射途徑輕松給藥，例如全身給藥（靜脈內(nèi)，動脈內(nèi)）或局部給藥（腫瘤內(nèi)，腦室內(nèi)）。

IN VIVO POLYMAG 500 轉(zhuǎn)染試劑的結(jié)果示例：

圖1：IN VIVO PolyMag 在靜脈注射后靶向基因遞送的高效率。熒光素酶表達42h后將與IN VIVO PolyMag納米顆粒復合的報告基因注射到兔耳動脈中。基因表達主要在磁體位置位點發(fā)現(xiàn)。作為對照，在不施用磁鐵的情況下將相同的載體組合物注入對側(cè)血管中。未發(fā)現(xiàn)顯著的報告基因表達。

圖2：在高度嚴格的條件下，IN VIVO PolyMag 在難以轉(zhuǎn)染的組織中遞送 DNA 的高效率。將DNA復合物和體內(nèi)PolyMag納米顆粒注射到不存在（A）或在磁場（B）影響下的大鼠的回腸中。48小時后進行X-Gal染色，在宏觀水平（上圖）和微觀水平（下圖）存在磁鐵（B）的情況下，揭示了高效的基因遞送。在沒有磁場的情況下，僅觀察到罕見的轉(zhuǎn)染事件（A）